MANIPULATION OF CORYNEBACTERIUM-GLUTAMICUM BY GENE DISRUPTION AND REPLACEMENT

被引：88

作者：

SCHWARZER, A ^{[1
]}

PUHLER, A ^{[1
]}

机构：

[1] UNIV BIELEFELD,LEHRSTUHL GENET,W-4800 BIELEFELD 1,GERMANY

来源：

BIO-TECHNOLOGY | 1991年 / 9卷 / 01期

关键词：

D O I：

10.1038/nbt0191-84

中图分类号：

Q81 [生物工程学（生物技术）]; Q93 [微生物学];

学科分类号：

071005 ; 0836 ; 090102 ; 100705 ;

摘要：

We have developed a system for the genetic manipulation of the amino acid-producing Corynebacterium glutamicum. Gene disruption and replacement were achieved by intoducing, via conjugation, Escherichia coli vector plasmids carrying manipulated C. glutamicum DNA fragments. We obtained stable mutants in which the chromosomal lysA gene, encoding meso-diaminopimelate decarboxylase, was interrupted by a chloramphenicol resistance cartridge, or in which an essential internal part of the lysA gene was deleted. The deletion mutants retain neither antibiotic resistance markers nor vector sequences. This strategy is generally applicable to the construction of industrial strains to be used in fermentation processes.

引用

页码：84 / 87

页数：4

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