FERTILIZATION ABNORMALITIES IN HUMAN INVITRO FERTILIZATION

被引：96

作者：

PLACHOT, M

CROZET, N

机构：

[1] U 173 Inscrm, Hôpital Neckcr, 75743

[2] INRA Unité Biologie de la Fécondation

来源：

HUMAN REPRODUCTION | 1992年 / 7卷

关键词：

CHROMOSOMES; MITOTIC SPINDLE; TRIPLOIDY; HAPLOIDY; HUMAN OOCYTES; HUMAN EMBRYOS;

D O I：

10.1093/humrep/7.suppl_1.89

中图分类号：

R71 [妇产科学];

学科分类号：

100211 ;

摘要：

Fertilization abnormalities (premature chromosome condensation of spermatozoa (PCC), triploidy, haploidy) were analysed in order to determine their origin. PCC occurs in 9% of unfertilized oocytes and seems to be the consequence of a failure of oocyte activation, leading to the continuing presence of cytoplasmic chromosome-condensing factors, causing the sperm nucleus to undergo chromosome condensation prematurely. This anomaly appears to be related to incomplete nuclear and/or cytoplasmic maturation. Triploid zygotes (6.5% of fertilized oocytes) display an original type of division: half of them divide into 3 and 6 cells, whereas at the same time diploid zygotes divide into 2 and then 4 cells. A cytological study, using both antitubulin antibodies and Hoechst dye, allowed us to demonstrate that they divide into 3 cells by means of a tripolar spindle. Triploidy seems to be correlated with four of 16 clinical or biological parameters examined: semen origin (fresh or frozen), type of stimulation treatment, number of oocytes recovered and embryo morphology. Haploid eggs (1.6% of inseminated oocytes) result from parthenogenetic activation. A correlation was found between a high number of recovered oocytes and triploid zygotes, and the occurrence of oocyte activation. These data show that increasing follicular recruitment decreases the overall oocyte quality and maturity leading to an overall 9% with impaired fortilization.

引用

页码：89 / 94

页数：6

共 30 条

[1]

Angel] R.R., Templeton A.A., Messinis I.E., Consequences of polyspermy in man, Cytogenet. Cell Genet., 42, pp. 1-7, (1986)

[2]

Angell R.R., Chromosome abnormalities in human preimplantation embryos, Development of Preimplantation Em-Bryos and Their Environment, pp. 181-187, (1989)

[3]

Angell R.R., Ledger W., Yong E.L., Harkness L., Baird D.T., Cytogenetic analysis of unfertilized human oocytes, Hum. Reprod., 6, pp. 568-573, (1991)

[4]

Calafell J.M., Badenas J., Catala V., Egozcue J., Santalo J., Premature chromosome condensation (PCC) as a sign of oocyte im-maturity. Hum. Reprod, Abstracts of The, 2, (1990)

[5]

Dandekar P.V., Martin M.C., Glass R.H., Polynuclear embryos after in vitro fertilization, Fertil. Steril., 53, pp. 510-514, (1990)

[6]

Delhanty J.D.A., Penketh R.L.A., Cytogenetic analysis of unfer-tilized oocytes retrieved after treatment with the LHRH analogue, buserelin, Hum. Reprod., 5, pp. 699-702, (1990)

[7]

Janny L., Benkhalifa M., Pouly J.L., Vye P., Canis M., Geneix A., Malet P., Boucher D., La condensation prématurée des chro-mosomes: Facteur fréquent d’échec en fécondation in vitro? Contr, Fert, Sex., 18, pp. 536-537, (1990)

[8]

Johnson R.T., Rao P.N., Mammalian cell fusion: Induction of premature chromosome condensation in interphase nuclei, Nature, 226, pp. 717-722, (1970)

[9]

Johnson M.H., Pickering S.J., Braude P.R., Vincent C., Cant A., Currie J., Acid Tyrode’s solution can stimulate parthenogenetic activation of human and mouse oocytes, Fertil. Steril., 53, pp. 266-270, (1990)

[10]

Kola I., Trounson A., Dawson G., Rogers P., Tripronuclear human oocytes: Altered cleavage patterns and subsequent karyotypic analysis of embryos, Biol. Reprod., 37, pp. 395-401, (1987)

← 1 2 3 →