ANCHORING OF DNA TO THE BACTERIAL CYTOPLASMIC MEMBRANE THROUGH COTRANSCRIPTIONAL SYNTHESIS OF POLYPEPTIDES ENCODING MEMBRANE-PROTEINS OR PROTEINS FOR EXPORT - A MECHANISM OF PLASMID HYPERNEGATIVE SUPERCOILING IN MUTANTS DEFICIENT IN DNA TOPOISOMERASE-I

被引：131

作者：

LYNCH, AS ^{[1
]}

WANG, JC ^{[1
]}

机构：

[1] HARVARD UNIV, DEPT BIOCHEM & MOLEC BIOL, 7 DIVIN AVE, CAMBRIDGE, MA 02138 USA

来源：

JOURNAL OF BACTERIOLOGY | 1993年 / 175卷 / 06期

关键词：

D O I：

10.1128/JB.175.6.1645-1655.1993

中图分类号：

Q93 [微生物学];

学科分类号：

071005 ; 100705 ;

摘要：

A homologous set of plasmids expressing tet, lacY, and melB, genes encoding integral cytoplasmic membrane proteins, and tolC and ampC, genes encoding proteins for export through the cytoplasmic membrane, was constructed for studying the effects of transcription and translation of such genes on the hypernegative supercoiling of plasmids in Escherichia coli cells deficient in DNA topoisomerase I. The results support the view that intracellular bacterial DNA is anchored to the cytoplasmic membrane at many points through cotranscriptional synthesis of membrane proteins or proteins designated for export across the cytoplasmic membrane; in the latter case, the presence of the signal peptide appears to be unnecessary for cotranscriptional membrane association.

引用

页码：1645 / 1655

页数：11

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