BIOLOGICAL ALTERNATIVES TO CHEMICAL-IDENTIFICATION FOR THE ECOTOXICOLOGICAL ASSESSMENT OF INDUSTRIAL EFFLUENTS - THE RTG-2 IN-VITRO CYTOTOXICITY TEST

被引:15
作者
CASTANO, A [1 ]
VEGA, M [1 ]
BLAZQUEZ, T [1 ]
TARAZONA, JV [1 ]
机构
[1] INST SALUD CARLOS 3, CTR SANIDAD AMBIENTAL, MADRID, SPAIN
关键词
RTG-2; CELLS; TOXICITY; EFFLUENTS; BIOASSESSMENT;
D O I
10.1002/etc.5620131009
中图分类号
X [环境科学、安全科学];
学科分类号
08 ; 0830 ;
摘要
Ecotoxicology is concerned with the effects of chemicals on biological systems. Identifying components of complex aqueous effluents poses special problems, and can be useless if there is a lack of information on the biological effects of the identified chemicals. Toxicity-based (bioassay-directed) sample fractionation can be very useful, but the small amount of fractioned material is a constraint that can be solved by using in vitro tests. The RTG-2 in vitro cytotoxicity test has been used to assess (a) the efficacy of a treatment plant in the aeronautics industry and (b) the exposure of fish and molluscs cultured in Esteiro Bay to the effluent of a fish-processing factory. Ecotoxicological assessments could be done without identifying the responsible chemicals. The RTG-2 test was used in combination with concentration/fractionation procedures. It proved that the toxicity of the liquid wastes from the aeronautics industry was eliminated by the treatment, and that molluscs and fish reared in Esteiro Bay had accumulated toxic chemicals dumped by the fish-processing factory. A combination of the RTG-2 cytotoxicity test and HPLC proved to give useful information even for chemicals not identified by GC-MS.
引用
收藏
页码:1607 / 1611
页数:5
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