A BIOLOGIC ASSAY FOR IL-4 - RAPID FLUORESCENCE ASSAY FOR IL-4 DETECTION IN SUPERNATANTS AND SERUM

Interleukin-4 (IL-4) is a lymphokine produced by activated T helper cells with effects on T cells, B cells, monocytes and mast cells. The conventional tonsillar B cell assay used for quantification of IL-4 activity is sensitive to the presence of other cytokines and requires the acquisition of fresh cells on a regular basis. We have evaluated the ability of IL-4 in the presence of antibody to IgM to induce CD23 on a cultured B cell line (Ramos) The requirements of measuring hundreds of samples at a single time precluded ready use of a conventional flow cytometer. We therefore developed a rapid fluorescence assay which allows the detection of IL-4 in supernatants and serum. The use of a particle fluorescence immunoassay reproducibly allows detection of IL-4 to 6 U in supernatants and serum. This assay is specific for IL-4 and is not sensitive to other recombinant cytokines including IL-1, IL-2, IL-3, IL-6; interferon-α, -β or -γ, tumor necrosis factor (TNF) or GM-CSF. Finally, in cancer patients receiving IL-4 its detection in serum using this assay reveals an α distribution phase of approximately 8 min and a β clearance phase of approximately 48 min. © 1990.