NEOVASCULARIZATION OF PROSTHETIC VASCULAR GRAFTS - QUANTITATIVE-ANALYSIS OF ANGIOGENESIS AND MICROHEMODYNAMICS BY MEANS OF INTRAVITAL MICROSCOPY

被引:38
作者
MENGER, MD
HAMMERSEN, F
WALTER, P
MESSMER, K
机构
[1] TECH UNIV MUNICH,DEPT ANAT,W-8000 MUNICH 2,GERMANY
[2] UNIV HEIDELBERG,DEPT EXPTL SURG,W-6900 HEIDELBERG,GERMANY
关键词
Hamster; Intravital microscopy; Neovascularization; Prosthetic vascular grafts;
D O I
10.1055/s-2007-1014008
中图分类号
R5 [内科学];
学科分类号
1002 ; 100201 ;
摘要
Neovascularization of prosthetic vascular grafts seems to play an important role in the prevention of early graft failure due to infection or thrombotic occlusion. The process of angiogenesis ane neovascularization was analyzed for three different prosthetic vascular grafts (PTFE, Dacron double microvelour and gelatin-coated Dacron double microvelour) in vivo by means of fluorescence microscopy. Under Nembutal anesthesia (50 mg/kg BW) Syrian golden hamsters were fitted with a dorsal skinfold chamber, which contains the cutaneous skeletal muscle as well as subcutaneous tissue, and allows for quantitative analysis of the microcirculation for a prolonged period of time. In each chamber one piece (1 mm2) of all three vascular grafts was implanted. Five days after implantation neovascularization was ascertained in 9/11 (coated) and 8/11 (non-coated) Dacron grafts, while only 4/11 PTFE implants revealed new microvessels. On day 10 the density of newly-formed microvessels was significantly higher (p < 0.001) in Dacron grafts (234.3 ± 31.2 cm-1 and 238.9 ± 41.3 cm-1 respectively) as compared to PFTE implants (154.9 ± 30.4 cm-1). In addition, Dacron grafts revealed a larger neovascularization zone extending into the perigraft tissue. 12 days after implantation non-coated Dacron grafts were most tightly integrated into the perigraft tissue. The better neovascularization of Dacron might be due to the high porosity of the graft compared to low porous PTFE, which revealed insufficient neovascularization.
引用
收藏
页码:139 / 145
页数:7
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