PRIMARY STRUCTURE AND EXPRESSION OF BOVINE POLY(A) POLYMERASE

被引：139

作者：

RAABE, T ^{[1
]}

BOLLUM, FJ ^{[1
]}

MANLEY, JL ^{[1
]}

机构：

[1] UNIFORMED SERV UNIV HLTH SCI,DEPT BIOCHEM,BETHESDA,MD 20814

来源：

NATURE | 1991年 / 353卷 / 6341期

关键词：

D O I：

10.1038/353229a0

中图分类号：

O [数理科学和化学]; P [天文学、地球科学]; Q [生物科学]; N [自然科学总论];

学科分类号：

07 ; 0710 ; 09 ;

摘要：

Poly(A) polymerase has a critical role in the synthesis of messenger RNA in eukaryotic cells. The isolation and characterization of complementary DNAs encoding bovine poly(A) polymerase is described here. The predicted sequences of the MRNA and protein reveal features that provide insights into how the enzyme functions and how it might be regulated. Poly(A) polymerase expressed from a cloned cDNA is fully functional in in vitro assays, and mutational analyses have identified a putative regulatory domain that enhances, but is not essential for, activity.

引用

页码：229 / 234

页数：6

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