RECOMBINANT PROTEIN EXPRESSION IN HIGH CELL-DENSITY FED-BATCH CULTURES OF ESCHERICHIA-COLI

被引：206

作者：

YEE, L ^{[1
]}

BLANCH, HW ^{[1
]}

机构：

[1] UNIV CALIF BERKELEY, DEPT CHEM ENGN, BERKELEY, CA 94702 USA

来源：

BIO-TECHNOLOGY | 1992年 / 10卷 / 12期

关键词：

D O I：

10.1038/nbt1292-1550

中图分类号：

Q81 [生物工程学（生物技术）]; Q93 [微生物学];

学科分类号：

071005 ; 0836 ; 090102 ; 100705 ;

摘要：

Whereas cell concentrations of 5-10 grams dry cell weight per liter (gDCW/l) are typical of batch cultures, fed-batch techniques can be used to achieve concentrations greater than 50 gDCW/l. Feeding strategies for fed-batch cultures include feedback control as well as pre-determined feeding profiles. The volumetric yield of recombinant products can be improved by controlling the specific growth rate and the substrate concentration. Furthermore, inhibitory by-product formation can be minimized in fed-batch cultures. This review focuses on the use of fed-batch techniques to produce recombinant products in Escherichia coli. The modes of nutrient feeding that have been employed are discussed, and the factors important in attaining high cell concentrations as well as high specific yields of recombinant product are described.

引用

页码：1550 / 1556

页数：7

共 60 条

[1]

ALLEN B, 1987, BIOPHARM MANUF, V1, P38

[2] HIGH-CELL-DENSITY FERMENTATION STUDIES OF A RECOMBINANT ESCHERICHIA-COLI THAT EXPRESSES ATRIAL-NATRIURETIC-FACTOR [J].

BAILEY, FJ ;

BLANKENSHIP, J ;

CONDRA, JH ;

MAIGETTER, RZ ;

ELLIS, RW .

JOURNAL OF INDUSTRIAL MICROBIOLOGY, 1987, 2 (01) :47-52

[3] MAXIMAL EXPONENTIAL-GROWTH RATE AND YIELD OF ESCHERICHIA-COLI OBTAINABLE IN A BENCH-SCALE FERMENTOR [J].

BAUER, S ;

SHILOACH, J .

BIOTECHNOLOGY AND BIOENGINEERING, 1974, 16 (07) :933-941

[4] PILOT SCALE EXPONENTIAL-GROWTH OF ESCHERICHIA-COLI W TO HIGH CELL CONCENTRATION WITH TEMPERATURE-VARIATION [J].

BAUER, S ;

WHITE, MD .

BIOTECHNOLOGY AND BIOENGINEERING, 1976, 18 (06) :839-846

[5] DENSE GROWTH OF AEROBIC BACTERIA IN A BENCH-SCALE FERMENTOR [J].

BAUER, S ;

ZIV, E .

BIOTECHNOLOGY AND BIOENGINEERING, 1976, 18 (01) :81-94

[6] CONTINUOUS PRODUCTION OF HUMAN-LEUKOCYTE INTERFERON WITH ESCHERICHIA-COLI AND CONTINUOUS CELL-LYSIS IN A 2 STAGE CHEMOSTAT [J].

BROWN, SW ;

MEYER, HP ;

FIECHTER, A .

APPLIED MICROBIOLOGY AND BIOTECHNOLOGY, 1985, 23 (01) :5-9

[7] Observations on the carbohydrate metabolism of tumours. [J].

Crabtree, HG .

BIOCHEMICAL JOURNAL, 1929, 23 (03) :536-545

[8] DESIGN AND EVALUATION OF A 2-STAGE, CYCLIC, RECOMBINANT FERMENTATION PROCESS [J].

CURLESS, C ;

FU, K ;

SWANK, R ;

MENJARES, A ;

FIESCHKO, J ;

TSAI, L .

BIOTECHNOLOGY AND BIOENGINEERING, 1991, 38 (09) :1082-1090

[9] EFFECT OF PREINDUCTION SPECIFIC GROWTH-RATE ON RECOMBINANT ALPHA-CONSENSUS INTERFERON SYNTHESIS IN ESCHERICHIA-COLI [J].

CURLESS, C ;

POPE, J ;

TSAI, L .

BIOTECHNOLOGY PROGRESS, 1990, 6 (02) :149-152

[10] HIGH CELL-DENSITY CULTURE OF ESCHERICHIA-COLI IN A FED BATCH SYSTEM WITH DISSOLVED-OXYGEN AS SUBSTRATE FEED INDICATOR [J].

CUTAYAR, JM ;

POILLON, D .

BIOTECHNOLOGY LETTERS, 1989, 11 (03) :155-160

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