RAT PLEURAL AND PERITONEAL MAST-CELLS STIMULATED AT DIFFERENT CELLULAR-LEVELS - DIFFERENCE IN AND INFLUENCE OF PURIFICATION MEDIA

被引：10

作者：

CABADO, AG

VIEYTES, MR

BOTANA, LM

机构：

[1] FAC VET LUGO,DEPT FARMACOL,E-27002 LUGO,SPAIN

[2] FAC VET LUGO,DEPT FARMACOL,E-27002 LUGO,SPAIN

来源：

INTERNATIONAL ARCHIVES OF ALLERGY AND IMMUNOLOGY | 1993年 / 100卷 / 03期

关键词：

PURIFICATION MEDIA; NYCODENZ(R); PERCOLL(R); FICOLL(R); SUCROSE; BOVINE SERUM ALBUMIN; SODIUM FLUORIDE;

D O I：

10.1159/000236417

中图分类号：

R392 [医学免疫学];

学科分类号：

100102 ;

摘要：

We checked the effect that purification through different media can produce on rat mast cell response, considering different populations of pleural and peritoneal mast cells. We compared the media Ficoll(R), Percoll(R), bovine serum albumin and sucrose with the seldom used Nycodenz(R). Histamine release was elicited with compound 48/80, polymyxin B, ionophore A23187 and calcium on cells preincubated with FNa. High osmolarity media induced the most important changes on the cell response as compared to low osmolarity media. Our results show that Ficoll and sucrose induced the most notable changes in the response. Also, the more sensitive stimulus to the purification procedure is sodium fluoride-calcium. The response to ionophore A23187 did not show any important alteration.

引用

页码：234 / 239

页数：6

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