POLYMER PRODUCTION BY KLEBSIELLA-PNEUMONIAE 4-HYDROXYPHENYLACETIC ACID HYDROXYLASE GENES CLONED IN ESCHERICHIA-COLI

被引：20

作者：

GIBELLO, A

FERRER, E

SANZ, J

MARTIN, M

机构：

[1] UNIV COMPLUTENSE MADRID,FAC VET,DEPT BIOQUIM & BIOL MOLEC IV,E-28040 MADRID,SPAIN

[2] UNIV COMPLUTENSE MADRID,FAC VET,DEPT MICROBIOL PATOL ANIM 1,E-28040 MADRID,SPAIN

[3] CSIC,INST QUIM ORGAN,E-28006 MADRID,SPAIN

来源：

APPLIED AND ENVIRONMENTAL MICROBIOLOGY | 1995年 / 61卷 / 12期

关键词：

D O I：

10.1128/AEM.61.12.4167-4171.1995

中图分类号：

Q81 [生物工程学（生物技术）]; Q93 [微生物学];

学科分类号：

071005 ; 0836 ; 090102 ; 100705 ;

摘要：

The expression of Klebsiella pneumoniae hpaA and hpaH genes, which code for 4-hydroxyphenylacetic acid hydroxylase in Escherichia coli K-12 derivative strains, is associated with the production of a dark brown pigment in the cultures. This pigment has been identified as a polymer which shows several of the characteristics reported for microbial melanins and results from the oxidative activity of 4-hydroxyphenylacetic acid hydroxylase on some dihydroxylated compounds to form o-quinones. A dibenzoquinone is formed from the oxidation of different mono- or dihydroxylated aromatic compounds by the enzyme prior to polymerization. We report a hydroxylase activity, other than tyrosinase, that is associated with the synthesis of a bacterial melanin.

引用

页码：4167 / 4171

页数：5

共 35 条

[1] TRANSPORT OF 4-HYDROXYPHENYLACETIC ACID IN KLEBSIELLA-PNEUMONIAE [J].