THE HYPOOSMOTIC SWELLING TEST AND CRYOSURVIVAL OF HUMAN SPERMATOZOA

被引:16
作者
CHAN, SYW
CRAFT, IL
CHAN, YM
LEONG, MKH
LEUNG, CKM
机构
[1] IVF Centre, Hong Kong Sanatorium and Hospital, Happy Valley
[2] Fertility and IVF Unit, Humana Hospital Wellington, London, Wellington Place
关键词
Cryopreservation; Cryosurvival; Human spermatozoa; Hypostomatic swelling;
D O I
10.1093/oxfordjournals.humrep.a137174
中图分类号
R71 [妇产科学];
学科分类号
100211 ;
摘要
The hypoosmotic swelling test is a simple laboratory test to evaluate the functional integrity of the membrane of human spermatozoa. This test was performed on 83 samples of human semen before cryopreservation to determine whether it has any predictive value for the cryosurvival of human spermatozoa. Stepwise regression analysis demonstrated that conventional sperm characteristics, including the concentration, motility, normal morphology and viability, of pre-freeze semen samples were of limited value in predicting the cryosurvival of human spermatozoa. Further, the hypoosmotic swelling test results from pre-freeze semen samples did not correlate with the post-thaw motility or the survival rate of spermatozoa after cryopreservation. © 1990 Oxford University Press.
引用
收藏
页码:715 / 718
页数:4
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