CA2+-INDEPENDENT CONTRACTION OF UTERINE SMOOTH-MUSCLE INDUCED BY VANADATE AND ITS INHIBITION BY CA2+

被引：10

作者：

FUKUZAKI, A ^{[1
]}

SUGA, O ^{[1
]}

KARIBE, H ^{[1
]}

MIYAUCHI, Y ^{[1
]}

GOKITA, T ^{[1
]}

UCHIDA, MK ^{[1
]}

机构：

[1] MEIJI COLL PHARM,DEPT MOLEC PHARMACOL,1-35-23 NOZAWA,SETAGAYA KU,TOKYO 154,JAPAN

来源：

EUROPEAN JOURNAL OF PHARMACOLOGY | 1992年 / 220卷 / 01期

关键词：

VANADATE; UTERUS; SMOOTH MUSCLE; CA2+; CA2+-FREE; PROTEIN KINASE-C;

D O I：

10.1016/0014-2999(92)90018-Y

中图分类号：

R9 [药学];

学科分类号：

1007 ;

摘要：

Vanadate, 30-mu-M, contracts uterine smooth muscle of estrogen-dominated non-pregnant rats in Ca2+-free medium after preincubation with 3 mM EGTA. In spite of the phosphorylation of the myosin light chain during this contraction, studies with fura-2 suggested that this contraction was not accompanied by an increase in the cytosolic Ca2+ level. Inhibitors of the myosin light chain kinase and protein kinase C partly inhibited this contraction. Vanadate seems to enter the cell through anion channels to inhibit phosphatases, resulting in phosphorylation via basal activities of the myosin light chain kinase and protein kinase C. An increase in the cytosolic free Ca2+ level resulted in relaxation of the contracting muscle in the same manner as in the oxytocin-induced Ca2+-free contraction.

引用

页码：99 / 102

页数：4

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