MAPPING THE CLOCK RHYTHM MUTATION TO THE PERIOD LOCUS OF DROSOPHILA-MELANOGASTER BY GERMLINE TRANSFORMATION

被引:18
作者
DUSHAY, MS
ROSBASH, M
HALL, JC
机构
[1] BRANDEIS UNIV,DEPT BIOL,WALTHAM,MA 02254
[2] BRANDEIS UNIV,HOWARD HUGHES MED INST,WALTHAM,MA 02254
关键词
CIRCADIAN RHYTHMS; LOCOMOTOR ACTIVITY; P-ELEMENT TRANSGENICS;
D O I
10.3109/01677069209083446
中图分类号
Q3 [遗传学];
学科分类号
071007 ; 090102 ;
摘要
The Clock (Clk) mutation shortens circadian rhythms of locomotor activity and eclosion from ca. 24 h to 22.5-23 h. Clk was previously mapped, by meiotic recombination, very close to the period(per) locus on the X chromosome. To determine whether Clk is a mutation within the per gene or if the former is separate from the latter, two overlapping genomic fragments were cloned from Clk flies to produce a per-containing 13.2 kb construct. per01 flies (which by themselves are arrhythmic)-when transformed with this construct-expressed short-period rhythms. This indicates that the Clk mutation is contained within this 13.2 kb region and is almost certainly a new "fast-clock" allele of per.
引用
收藏
页码:173 / 179
页数:7
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