LOCALIZATION OF CIS-ACTING SEQUENCES ESSENTIAL FOR CYMBIDIUM RINGSPOT TOMBUSVIRUS DEFECTIVE INTERFERING RNA REPLICATION

被引:28
作者
HAVELDA, Z [1 ]
DALMAY, T [1 ]
BURGYAN, J [1 ]
机构
[1] AGR BIOTECHNOL CTR, INST PLANT SCI, H-2101 GODOLLO, HUNGARY
关键词
D O I
10.1099/0022-1317-76-9-2311
中图分类号
Q81 [生物工程学(生物技术)]; Q93 [微生物学];
学科分类号
071005 ; 0836 ; 090102 ; 100705 ;
摘要
The smallest defective interfering RNA (DI-2) of cymbidium ringspot tombusvirus (CyRSV) was used to identify the cis-acting sequences necessary for its replication by making a series of deletions throughout the 404 nt long molecule and testing the biological activity of mutants. Deletion or substitution of the conserved sequence blocks (A, B and C) always yielded inactive molecules. The deletion of only a few nucleotides could be tolerated beyond the natural deletion sites in blocks A and B. However, either half of block C1 (34 nt) and the first 25 nt of C2 (102 nt) could be deleted without loss of infectivity. It was also demonstrated that either one of the two halves of block C1 was specifically required for replication. We suggest that the last 77 nt of the viral genome and either half of block C1 represent the complementary strand promoter sequence recognized by the viral replicase.
引用
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页码:2311 / 2316
页数:6
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