TRAPPING DNA-POLYMERASES USING TRIPLEX-FORMING OLIGODEOXYRIBONUCLEOTIDES

被引：39

作者：

SAMADASHWILY, GM ^{[1
]}

MIRKIN, SM ^{[1
]}

机构：

[1] UNIV ILLINOIS,DEPT GENET,CHICAGO,IL 60612

来源：

GENE | 1994年 / 149卷 / 01期

关键词：

DNA POLYMERIZATION; TERMINATION; TRIPLEX;

D O I：

10.1016/0378-1119(94)90421-9

中图分类号：

Q3 [遗传学];

学科分类号：

071007 ; 090102 ;

摘要：

Triplexes (triple helices) formed within DNA templates prior to or during DNA synthesis cause DNA polymerase to terminate [Samadashwily et al., EMBO J. 13 (1993) 4975-4983]. Here, we show that tripler-forming oligodeoxyribonucleotides (oligos) efficiently trap DNA polymerases at target DNA sequences within single-stranded (ss) templates. This was observed for all studied DNA polymerases, including Sequenase and the thermophilic Taq and Vent polymerases. The termination rate depends on the fine structure of a tripler, as well as on ambient conditions such as temperature and the concentration of magnesium ions. Inhibition of DNA synthesis was observed not only when triplexes blocked the path of DNA polymerase, but also when a polymerization primer was involved in tripler formation. Escherichia coli ss-binding (SSB) protein helps DNA polymerase overcome the tripler barrier, but with an efficiency dramatically dependent on the tripler configuration. These results describe a novel method for blocking DNA replication at target homopurine-homopyrimidine sequences by means of tripler-forming oligos in direct analogy with similar results during transcription.

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收藏

页码：127 / 136

页数：10

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