INVOLVEMENT OF PROTEIN-KINASE-C IN THE REGULATION OF OOCYTE MATURATION IN AMPHIBIANS (RANA-DYBOWSKII)

被引:30
作者
KWON, HB
LEE, WK
机构
[1] Chonnam National University, College of Natural Sciences, Department of Biology, Kwangju
来源
JOURNAL OF EXPERIMENTAL ZOOLOGY | 1991年 / 257卷 / 01期
关键词
D O I
10.1002/jez.1402570115
中图分类号
Q95 [动物学];
学科分类号
071002 ;
摘要
Ovarian oocytes of Rana dybowskii, isolated early in the hibernation period (late autumn), failed to mature, i.e., germinal vesicle breakdown (GVBD), in response to progesterone during in vitro follicle culture. Oocytes collected during the middle hibernation period matured in response to progesterone, whereas those collected late during the hibernation period (close to the breeding season) underwent spontaneous maturation without added hormone (Kwon et al., '89). The maturational response (GVBD) of oocytes, collected at the three stages of hibernation, to protein kinase C (PKC) activation was investigated and compared to that of progesterone stimulation. A phorbol ester, phorbol 12-myristate 13-acetate (TPA) was used for PKC activation. TPA addition to cultured follicles collected during the early or middle period of hibernation induced oocyte GVBD. The incidence of maturation (% GVBD) induced by TPA varied markedly between animals. TPA (10-mu-M) induced oocyte maturation in the presence or absence of follicle cells. The time course of the TPA-induced maturation was similar to that of progesterone-stimulated maturation (ED50, 7-9 h). TPA also accelerated the onset of maturation of the follicular oocytes exhibiting spontaneous in vitro maturation. Both TPA- and progesterone-stimulated maturation was blocked by treatment with cycloheximide(1-mu-g/2 ml), forskolin (9-mu-M) (an adenylate cyclase stimulator), and verapamil (0.27 mM) (a calcium transport blocker). Treatment of oocytes with a calmodulin antagonist N-(6-aminohexyl)-5-chloro-1-naphthalenesulfonamide (W-7) (100-mu-M) or a PKC inactivator 1-(5-isoquinolinylsulfonyl)-2-methyl-piperazine (H-7) (50-mu-M) likewise suppressed TPA- or progesterone-induced maturation. The data suggest that PKC plays a role in the regulation of amphibian oocyte maturation and that the seasonal change in responsiveness (GVBD) of oocytes to steroid occurs after oocytes obtain responsiveness to PKC activation.
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页码:115 / 123
页数:9
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