PHOSPHOROTHIOATE OLIGONUCLEOTIDES BIND IN A NON SEQUENCE-SPECIFIC MANNER TO THE NUCLEOLAR PROTEIN C23/NUCLEOLIN

被引：61

作者：

WEIDNER, DA ^{[1
]}

VALDEZ, BC ^{[1
]}

HENNING, D ^{[1
]}

GREENBERG, S ^{[1
]}

BUSCH, H ^{[1
]}

机构：

[1] BAYLOR COLL MED,DEPT PHARMACOL,HOUSTON,TX 77030

来源：

FEBS LETTERS | 1995年 / 366卷 / 2-3期

关键词：

PHOSPHOROTHIOATE OLIGONUCLEOTIDE; C23/NUCLEOLIN; RNA RECOGNITION MOTIF;

D O I：

10.1016/0014-5793(95)00517-D

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

To design optimal strategies for intracellular delivery of antisense phosphorothioate oligonucleotides, it may be useful to understand their interaction with cellular macromolecules. Nuclear extracts from LOX amelanotic myeloma cells were studied for protein binding to phosphorothioate oligonucleotides using a Southwestern protocol. Multiple nuclear proteins bound to the phosphorothioate oligonucleotides but no detectable protein binding was found to phosphodiester oligonucleotides. The protein with the strongest binding signals was shown by immunoprecipitation to be nucleolar C23/nucleolin, a 110 kDa protein. With glutathione S-transferase/nucleolin fusion protein constructs, the region of nucleolin containing the RNA recognition motifs had binding activity to phosphorothioate oligonucleotides.

引用

页码：146 / 150

页数：5

共 37 条

[1]

Melani, Rivoltini, Parmiani, Calabretta, Colombo, Cancer Res., 51, pp. 2897-2901, (1991)

[2]

Mirabelli, Bennett, Anderson, Crooke, Anti-Cancer Drug Design, 6, pp. 647-661, (1991)

[3]

Vickers, Baker, Cool, Zounes, Buckheit, Germany, Ecker, Nucleic Acids Res., 19, pp. 3359-3368, (1991)

[4]

Monia, Johnston, Ecker, Zounes, Lima, Freir, J. Biol. Chem., 267, pp. 19954-19962, (1992)

[5]

Stein, Cheng, Science, 261, pp. 1004-1012, (1993)

[6]

Loke, Stein, Zhang, Mori, Nakanishi, Subasinghe, Cohen, Netkais, Proc. Natl. Acad. Sci. USA, 86, pp. 3474-3478, (1989)

[7]

Yakubov, Deeva, Zarytova, Ivanova, Ryte, Yurchenko, Vlassov, Proc. Natl. Acad. Sci. USA, 86, pp. 6454-6458, (1989)

[8]

Geselowitz, Neckers, Antisense Res. Dev., 2, pp. 17-25, (1992)

[9]

Chin, Green, Zon, Szoka, Straubinger, New Biol., 2, pp. 1091-1100, (1990)

[10]

Leonetti, Mechti, Degols, Gagnor, Lebleu, Proc. Natl. Acad. Sci. USA, 88, pp. 2702-2706, (1991)

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