SIMULTANEOUS LOCAL PRESSURE MICROEJECTION OF EXCITATORY AMINO-ACIDS AND FIELD POTENTIAL RECORDING WITH A SINGLE MICROPIPETTE IN THE HIPPOCAMPAL SLICE

被引:9
作者
FEDOROV, NB [1 ]
REYMANN, KG [1 ]
机构
[1] FED INST NEUROBIOL,DEPT NEUROPHYSIOL,O-3010 MAGDEBURG,GERMANY
关键词
EXCITATORY AMINO ACIDS; HIPPOCAMPUS; PRESSURE MICROEJECTION; LOCAL FIELD RESPONSE; MICROIONTOPHORESIS; TETRODOTOXIN; STRETCH-ACTIVATED CHANNEL;
D O I
10.1016/0165-0270(93)90058-Y
中图分类号
Q5 [生物化学];
学科分类号
071010 ; 081704 ;
摘要
The ability to use the same micropipette for simultaneous pressure microejection and field potential recording ('spot pressure electrode') is described. This approach allows the use of a single electrode to record bath field responses to pressure microejection of drugs (fPR) and synaptically evoked field excitatory postsynaptic potentials (fEPSP). To demonstrate this, the glutamate receptor agonists alpha-amino-3-hydroxy-5-methyl-4-isoxazole propionate (AMPA, 100-300 mu M) and N-methyl-D-aspartate (NMDA, 1 mM) were used. Both AMPA- and NMDA-evoked fPRs exhibited properties of postsynaptic responses. Thus, they were not significantly altered by tetrodotoxin (TTX, 0.5-1 mu M), but were completely and reversibly blocked by an antagonist of AMPA/kainate receptors, 6-cyano-7-nitroquinoxaline-2,3-dione (CNQX, 20 mu M) and D-2-amino-5-phosphonopentanoic acid (AP5, 20 mu M), respectively. The responses to micropressure-ejected AMPA were more stable and probably restricted to a smaller locus than responses to iontophoretic AMPA ejection. Additionally, the AMPA and NMDA contained in the pipette (up to 500 mu M) did not influence the fEPSP recorded with this pipette. Our results suggest that this method might be a useful experimental tool for studying the local effects of excitatory amino acids and other compounds.
引用
收藏
页码:83 / 90
页数:8
相关论文
共 18 条
[1]   COMBINING INVIVO VOLUME-CONTROLLED PRESSURE MICROEJECTION WITH EXTRACELLULAR UNIT RECORDING [J].
AKAOKA, H ;
SAUNIER, CF ;
CHERGUI, K ;
CHARLETY, P ;
BUDA, M ;
CHOUVET, G .
JOURNAL OF NEUROSCIENCE METHODS, 1992, 42 (1-2) :119-128
[2]   FEEDFORWARD DENDRITIC INHIBITION IN RAT HIPPOCAMPAL PYRAMIDAL CELLS STUDIED INVITRO [J].
ALGER, BE ;
NICOLL, RA .
JOURNAL OF PHYSIOLOGY-LONDON, 1982, 328 (JUL) :105-123
[3]   SYNCHRONIZED GABAERGIC IPSPS RECORDED IN THE NEOCORTEX AFTER BLOCKADE OF SYNAPTIC TRANSMISSION MEDIATED BY EXCITATORY AMINO-ACIDS [J].
ARAM, JA ;
MICHELSON, HB ;
WONG, RKS .
JOURNAL OF NEUROPHYSIOLOGY, 1991, 65 (05) :1034-1041
[4]   MECHANOTRANSDUCING ION CHANNELS IN ASTROCYTES [J].
BOWMAN, CL ;
DING, JP ;
SACHS, F ;
SOKABE, M .
BRAIN RESEARCH, 1992, 584 (1-2) :272-286
[5]  
Curtis D. R., 1964, ELECTROPHYSIOLOGICAL, P144
[6]   NONPHARMACOLOGICAL EFFECTS OF THE USE OF MICROELECTROPHORESIS AND PRESSURE EJECTION OF DRUGS IN COMBINATION [J].
DAVIES, SN ;
CHURCH, J ;
LODGE, D .
JOURNAL OF NEUROSCIENCE METHODS, 1987, 20 (03) :221-227
[7]   TEMPORALLY DISTINCT PRE-SYNAPTIC AND POST-SYNAPTIC MECHANISMS MAINTAIN LONG-TERM POTENTIATION [J].
DAVIES, SN ;
LESTER, RAJ ;
REYMANN, KG ;
COLLINGRIDGE, GL .
NATURE, 1989, 338 (6215) :500-503
[8]   EFFECTS OF PHILANTHOTOXIN-343 ON CA1 PYRAMIDAL NEURONS OF RAT HIPPOCAMPUS INVITRO [J].
FEDOROV, NB ;
SCREBITSKY, VG ;
REYMANN, KG .
EUROPEAN JOURNAL OF PHARMACOLOGY-ENVIRONMENTAL TOXICOLOGY AND PHARMACOLOGY SECTION, 1992, 228 (04) :201-206
[9]  
GREGERSEN H, 1992, EUR J NEUROSCI, V5, P2177
[10]  
JOHNSON SW, 1986, J NEUROSCI, V6, P572