OLIGONUCLEOTIDE PRIMERS FOR DNA AMPLIFICATION OF THE EARLY REGION-1, REGION-6, AND REGION-7 FROM HUMAN PAPILLOMAVIRUS TYPE-6, TYPE-11, TYPE-16, TYPE-18, TYPE-31, AND TYPE-33

被引：33

作者：

EVANDER, M

BODEN, E

BJERSING, L

RYLANDER, E

WADELL, G

机构：

[1] UMEA UNIV, DEPT VIROL, S-90185 UMEA, SWEDEN

[2] UMEA UNIV HOSP, DEPT OBSTET & GYNECOL, S-90185 UMEA, SWEDEN

[3] UMEA UNIV, DEPT PATHOL, S-90185 UMEA, SWEDEN

来源：

ARCHIVES OF VIROLOGY | 1991年 / 116卷 / 1-4期

关键词：

D O I：

10.1007/BF01319244

中图分类号：

Q93 [微生物学];

学科分类号：

071005 ; 100705 ;

摘要：

Human papillomavirus (HPV) type-specific sequences required for polymerase chain reaction (PCR) mediated amplification of HPV DNA sequences are presented. One primer pair within the E1 open reading frame (ORF) was shared by HPV 6, HPV 11, HPV 16, and HPV 31, whereas the other primer pair within the E1 ORF was specific for HPV 16. Eight primer pairs from the E6 and E7 ORFs specifically detected HPV 6, HPV 16, HPV 18, and HPV 33 sequences. This system has been used for detection of HPV DNA in biopsies, cytological smears and sections of formalin-fixed tissues.

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页码：221 / 233

页数：13

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