GEL-PHASE P-31-NMR - A NEW ANALYTICAL TOOL TO EVALUATE SOLID-PHASE OLIGONUCLEOTIDE SYNTHESIS

被引：17

作者：

BARDELLA, F

ERITJA, R

PEDROSO, E

GIRALT, E

机构：

[1] UNIV BARCELONA,FAC QUIM,DEPT QUIM ORGAN,MARTI & FRANQUES 1-11,E-08028 BARCELONA,SPAIN

[2] CSIC,CID,DEPT GENET MOLEC,E-08034 BARCELONA,SPAIN

来源：

BIOORGANIC & MEDICINAL CHEMISTRY LETTERS | 1993年 / 3卷 / 12期

关键词：

GEL-PHASE NMR; OLIGONUCLEOTIDE SYNTHESIS; PHOSPHOTRIESTER METHOD; PHOSPHITE-TRIESTER METHOD; POLYSTYRENE;

D O I：

10.1016/S0960-894X(01)80766-6

中图分类号：

R914 [药物化学];

学科分类号：

100701 ;

摘要：

This paper shows Gel-phase P-31-NMR spectra of synthetic intermediates obtained during solid-phase oligonucleotide synthesis on polystyrene for the first time. We have demonstrated the application of this technique using the phosphotriester, H-phosphonate and phosphite triester approaches. The use of Gel-phase P-31-NMR for monitoring solid phase oligonucleotide synthesis is discussed.

引用

页码：2793 / 2796

页数：4

共 13 条

[1] COMPARATIVE-STUDY OF SUPPORTS FOR SOLID-PHASE COUPLING OF PROTECTED-PEPTIDE SEGMENTS [J].