IN VIVO-LIKE ANTIGENIC SURFACE-PROPERTIES OF STAPHYLOCOCCUS-AUREUS FROM BOVINE MASTITIS INDUCED UPON GROWTH IN MILK WHEY

被引:7
作者
MAMO, W
FROMAN, G
机构
[1] SWEDISH UNIV AGR SCI,BMC,DEPT VET MICROBIOL,BACTERIOL & EPIZOOTOL SECT,S-75123 UPPSALA,SWEDEN
[2] UNIV UPPSALA,INST CLIN BACTERIOL,S-75122 UPPSALA,SWEDEN
关键词
STAPHYLOCOCCUS AUREUS; BOVINE MASTITIS; MILK WHEY; IN VIVO SURFACE ANTIGEN;
D O I
10.1111/j.1348-0421.1994.tb01860.x
中图分类号
R392 [医学免疫学]; Q939.91 [免疫学];
学科分类号
100102 ;
摘要
Antigenic surface properties of Staphylococcus aureus strains grown in milk whey were compared with TSB-grown bacteria using immune-gold electron microscopy. It is shown that colloidal gold (CG) particles coated with polyclonal antibody raised against Staphylococcus aureus surface antigen expressed in vivo bound to the surface of S. aureus strain F1440 grown in milk whey, but not to homologous bacteria grown in TSB. S. aureus strains grown in milk whey agglutinated in the presence of the polyclonal antibody, whereas the corresponding bacteria grown in TSB did not agglutinate. Immune-gold particles did not bind to milk whey-grown bacteria treated with periodate. Periodate-treated milk whey-grown bacteria did not agglutinate in the presence of the polyclonal antibody, whereas periodate treatment had no effect on TSB-grown bacteria.
引用
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页码:801 / 804
页数:4
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