AMINOACYL DERIVATIVES OF NUCLEOSIDES, NUCLEOTIDES AND POLYNUCLEOTIDES .47. EFFECT OF NUCLEOTIDE SUBSTITUTION ON THE PEPTIDYLTRANSFERASE ACTIVITY OF 2'(3')-O-(AMINOACYL) OLIGONUCLEOTIDES

被引：11

作者：

TEZUKA, M ^{[1
]}

CHLADEK, S ^{[1
]}

机构：

[1] MICHIGAN CANC FDN,DETROIT,MI 48201

来源：

BIOCHEMISTRY | 1990年 / 29卷 / 03期

关键词：

D O I：

10.1021/bi00455a011

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Seven 2′(3′)-O-(aminoacyl) trinucleotides with structures derived from the 3′-terminal C-C-A sequence of aa-tRNA via nucleotide substitutions were investigated as acceptor substrates in the peptidyltransferase reaction and as inhibitors of substrate binding to the peptidyltransferase A site. It was found that all tested compounds were active in both systems, although substitution in the first and second nucleotide position results in some decrease of acceptor activity. Remarkably, replAcc.ment of natural cytidylic acid residues in C-C-A-Phe with guanylic acid moieties resulted only in a small decrease of acceptor or binding activity. The results indicate that the acceptor sequence of aa-tRNA is not probably engaged in base pairing with a sequence of 23S RNA during its interaction with the peptidyltransferase A site. © 1990, American Chemical Society. All rights reserved.

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页码：667 / 670

页数：4

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