PURIFICATION AND CHARACTERIZATION OF A GLUCOAMYLASE FROM HUMICOLA-GRISEA

被引：38

作者：

CAMPOS, L ^{[1
]}

FELIX, CR ^{[1
]}

机构：

[1] UNIV BRASILIA, DEPT BIOL CELULAR, BR-70910900 BRASILIA, DF, BRAZIL

来源：

APPLIED AND ENVIRONMENTAL MICROBIOLOGY | 1995年 / 61卷 / 06期

关键词：

D O I：

10.1128/AEM.61.6.2436-2438.1995

中图分类号：

Q81 [生物工程学（生物技术）]; Q93 [微生物学];

学科分类号：

071005 ; 0836 ; 090102 ; 100705 ;

摘要：

A thermostable extracellular glucoamylase from the thermophilic fungus Humicola grisea was purified to homogeneity. Its molecular mass and isoelectric point were 74 kDa and 8.4, respectively. The enzyme contained 5% carbohydrate, showed maximal activities at pH 6.0 and 60 degrees C, and was stable at 55 degrees C and pH 6.0 for 2 h. The K-m of soluble starch hydrolysis at 50 degrees C and pH 6.0 was 0.14 mg/ml. The purified enzyme was remarkably insensitive to glucose.

引用

页码：2436 / 2438

页数：3

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