SHUTTLE MUTAGENESIS OF LEGIONELLA-PNEUMOPHILA - IDENTIFICATION OF A GENE ASSOCIATED WITH HOST-CELL CYTOPATHICITY

被引：23

作者：

ARROYO, J

HURLEY, MC

WOLF, M

MCCLAIN, MS

EISENSTEIN, BI

ENGLEBERG, NC

机构：

[1] UNIV MICHIGAN,SCH MED,DEPT MICROBIOL & IMMUNOL,ANN ARBOR,MI 48109

[2] UNIV MICHIGAN,SCH MED,DEPT INTERNAL MED,ANN ARBOR,MI 48109

来源：

INFECTION AND IMMUNITY | 1994年 / 62卷 / 09期

关键词：

D O I：

10.1128/IAI.62.9.4075-4080.1994

中图分类号：

R392 [医学免疫学]; Q939.91 [免疫学];

学科分类号：

100102 ;

摘要：

We performed shuttle mutagenesis of Legionella pneumophila. Mutants were screened for reduced cellular infectivity. Approximately 10% of the mutants had decreased cytopathicity. The DNA sequence of one locus was determined; the inferred amino acid sequence revealed homology with transport proteins including Escherichia coli To!C, Bordetella pertussis CyaE, and Alcaligenes eutrophus CzcC and CnrC.

引用

页码：4075 / 4080

页数：6

共 35 条

[1] PHOA GENE FUSIONS IN LEGIONELLA-PNEUMOPHILA GENERATED INVIVO USING A NEW TRANSPOSON, MUDPHOA
ALBANO, MA
ARROYO, J
EISENSTEIN, BI
ENGLEBERG, NC
[J]. MOLECULAR MICROBIOLOGY, 1992, 6 (13) : 1829 - 1839
[2] ALBANO MP, UNPUB
[3] ARBER W, 1983, LAMBDA, V2, P433
[4] ARROYO J, 1993, LEGIONELLA, P85
[5] ARROYO J, 1993, THESIS U MICHIGAN AN
[6] Ausubel FM., 1988, CURRENT PROTOCOLS MO
[7] A COMPLEMENTATION ANALYSIS OF RESTRICTION AND MODIFICATION OF DNA IN ESCHERICHIA COLI
BOYER, HW
ROULLAND.D
[J]. JOURNAL OF MOLECULAR BIOLOGY, 1969, 41 (03) : 459 - &
[8] A LEGIONELLA-PNEUMOPHILA GENE ENCODING A SPECIES-SPECIFIC SURFACE PROTEIN POTENTIATES INITIATION OF INTRACELLULAR INFECTION
CIANCIOTTO, NP
EISENSTEIN, BI
MODY, CH
TOEWS, GB
ENGLEBERG, NC
[J]. INFECTION AND IMMUNITY, 1989, 57 (04) : 1255 - 1262
[9] SITE-SPECIFIC MUTAGENESIS IN LEGIONELLA-PNEUMOPHILA BY ALLELIC EXCHANGE USING COUNTERSELECTABLE COLE1 VECTORS
CIANCIOTTO, NP
LONG, R
EISENSTEIN, BI
ENGELBERG, NC
[J]. FEMS MICROBIOLOGY LETTERS, 1988, 56 (02) : 203 - 207
[10] PURIFICATION AND CHARACTERIZATION OF AN EXTRACELLULAR PROTEASE OF LEGIONELLA-PNEUMOPHILA
DREYFUS, LA
IGLEWSKI, BH
[J]. INFECTION AND IMMUNITY, 1986, 51 (03) : 736 - 743

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