CHLORIDE-SENSITIVE CA2+ ENTRY BY HISTAMINE AND ATP IN HUMAN AORTIC ENDOTHELIAL-CELLS

被引:46
作者
HOSOKI, E [1 ]
IIJIMA, T [1 ]
机构
[1] AKITA UNIV,SCH MED,DEPT PHARMACOL,AKITA 010,JAPAN
来源
EUROPEAN JOURNAL OF PHARMACOLOGY-MOLECULAR PHARMACOLOGY SECTION | 1994年 / 266卷 / 03期
关键词
AORTIC ENDOTHELIAL CELL; CA2+; CONCENTRATION; INTRACELLULAR FREE; CL(-); EXTRACELLULAR; FURA-2; ENDOTHELIUM-DEPENDENT VASODILATOR;
D O I
10.1016/0922-4106(94)90129-5
中图分类号
R9 [药学];
学科分类号
1007 ;
摘要
The regulation of intracellular free Ca2+ concentration ([Ca2+](i)) was studied in cultured human aortic endothelial cells loaded with the fluorescent Ca2+ indicator fura-2. Histamine and ATP at concentrations of 10 mu M and higher produced a biphasic change in [Ca2+](i), which consisted of an initial transient elevation followed by a sustained elevation. Reduction of the extracellular Cl- concentration to 40 mM, or exposure to the Cl- channel antagonist N-phenylanthranilic acid selectively prevented the sustained response to histamine or ATP, but they did not affect the sustained response to the Ca2+ ionophore ionomycin. Elevation of extracellular Kf concentration to 90 mM had no influence on the sustained response to histamine or ATP. These results suggest that the sustained elevation of [Ca2+](i) in response to histamine and ATP is due to the Cl--sensitive entry of extracellular Ca2+ in cultured human aortic endothelial cells.
引用
收藏
页码:213 / 218
页数:6
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