ENDOTHELIN-3 LIKE IMMUNOREACTIVITY IN PLASMA OF PATIENTS WITH CIRRHOSIS OF THE LIVER

被引：35

作者：

GULBERG, V

GERBES, AL

VOLLMAR, AM

PAUMGARTNER, G

机构：

[1] UNIV MUNICH, KLINIKUM GROSSHADERN, DEPT MED 2, W-8000 MUNICH 2, GERMANY

[2] UNIV MUNICH, INST PHARMACOL TOXICOL & PHARM, W-8000 MUNICH 2, GERMANY

来源：

LIFE SCIENCES | 1992年 / 51卷 / 14期

关键词：

D O I：

10.1016/0024-3205(92)90519-U

中图分类号：

R-3 [医学研究方法]; R3 [基础医学];

学科分类号：

1001 ;

摘要：

A highly specific and sensitive radioimmunoassay (RIA) has been established for determination of endothelin-3 like immunoreactivity in human plasma to investigate its possible role in hemodynamic alterations due to liver disease. Crossreactivity with other endothelin isoforms was always below 4 %, the lower detection limit following extraction on Sep-Pak C18 cartridges was 0.5 pg/ml. The concentration of endothelin-3 (mean +/- SEM) was 4.16 +/- 0.56 pg/ml (n = 13) in plasma of patients with cirrhosis of the liver, three fold higher than in age matched controls (1.35 +/- 0.27 pg/ml, n = 12, p < 0.01). Plasma immunoreactivity was confirmed to be endothelin-3 related by reverse-phase HPLC. These data could suggest a role of plasma endothelin-3 in circulatory changes, as they occur in cirrhosis of the liver.

引用

收藏

页码：1165 / 1169

页数：5

相关论文

共 15 条

[1]

DEGOUVILLE ACL, 1990, J PHARMACOL EXP THER, V254, P1024

[2] LOW-DOSES OF ENDOTHELIN-3 ELICIT ENDOTHELIUM DEPENDENT VASODILATION WHICH ACCOMPANIES WITH ELEVATION OF CYCLIC-GMP [J].

FUKUDA, N ;

SOMA, M ;

IZUMI, Y ;

MINATO, M ;

WATANABE, Y ;

WATANABE, M ;

HATANO, M .

JAPANESE CIRCULATION JOURNAL-ENGLISH EDITION, 1991, 55 (06) :617-622

[3]

GERBES AL, 1991, HEPATO-GASTROENTEROL, V38, P360

[4] DEGRADATION AND CLEARANCE OF ATRIAL NATRIURETIC FACTORS (ANF) [J].

GERBES, AL ;

VOLLMAR, AM .

LIFE SCIENCES, 1990, 47 (14) :1173-1180

[5] ATRIAL NATRIURETIC FACTOR AND RENIN-ALDOSTERONE IN VOLUME REGULATION OF PATIENTS WITH CIRRHOSIS [J].

GERBES, AL ;

WERNZE, H ;

ARENDT, RM ;

RIEDEL, A ;

SAUERBRUCH, T ;

PAUMGARTNER, G .

HEPATOLOGY, 1989, 9 (03) :417-422

[6] THE HUMAN ENDOTHELIN FAMILY - 3 STRUCTURALLY AND PHARMACOLOGICALLY DISTINCT ISOPEPTIDES PREDICTED BY 3 SEPARATE GENES [J].

INOUE, A ;

YANAGISAWA, M ;

KIMURA, S ;

KASUYA, Y ;

MIYAUCHI, T ;

GOTO, K ;

MASAKI, T .

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 1989, 86 (08) :2863-2867

[7] MOLECULAR AND CELLULAR MECHANISM OF ENDOTHELIN REGULATION - IMPLICATIONS FOR VASCULAR FUNCTION [J].

MASAKI, T ;

KIMURA, S ;

YANAGISAWA, M ;

GOTO, K .

CIRCULATION, 1991, 84 (04) :1457-1468

[8] ABUNDANCE OF ENDOTHELIN-3 IN RAT INTESTINE, PITUITARY-GLAND AND BRAIN [J].

MATSUMOTO, H ;

SUZUKI, N ;

ONDA, H ;

FUJINO, M .

BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS, 1989, 164 (01) :74-80

[9] ENDOTHELIN-1 AND ENDOTHELIN-3 PLAY DIFFERENT ROLES IN ACUTE AND CHRONIC ALTERATIONS OF BLOOD-PRESSURE IN PATIENTS WITH CHRONIC-HEMODIALYSIS [J].

MIYAUCHI, T ;

SUZUKI, N ;

KURIHARA, T ;

YAMAGUCHI, I ;

SUGISHITA, Y ;

MATSUMOTO, H ;

GOTO, K ;

MASAKI, T .

BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS, 1991, 178 (01) :276-281

[10] PLASMA-CONCENTRATIONS OF ENDOTHELIN-1 AND ENDOTHELIN-3 ARE ALTERED DIFFERENTLY IN VARIOUS PATHOPHYSIOLOGICAL CONDITIONS IN HUMANS [J].

MIYAUCHI, T ;

SUGISHITA, Y ;

YAMAGUCHI, I ;

AJISAKA, R ;

TOMIZAWA, T ;

ONIZUKA, M ;

MATSUDA, M ;

KONO, I ;

YANAGISAWA, M ;

GOTO, K ;

SUZUKI, N ;

MATSUMOTO, H ;

MASAKI, T .

JOURNAL OF CARDIOVASCULAR PHARMACOLOGY, 1991, 17 :S394-S397