DIRECT EXPRESSION OF ACTIVE SPINACH GLYCOLATE OXIDASE IN ESCHERICHIA-COLI

被引：29

作者：

MACHEROUX, P

MULROONEY, SB

WILLIAMS, CH

MASSEY, V

机构：

[1] UNIV MICHIGAN,SCH MED,DEPT BIOL CHEM,ANN ARBOR,MI 48104

[2] DEPT VET AFFAIRS MED CTR,ANN ARBOR,MI

来源：

BIOCHIMICA ET BIOPHYSICA ACTA | 1992年 / 1132卷 / 01期

关键词：

GLYCOLATE OXIDASE; ENZYME EXPRESSION; EXPRESSION COMPARISON; (ESCHERICHIA-COLI);

D O I：

10.1016/0167-4781(92)90046-3

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Spinach glycolate oxidase (GAO) was expressed in Escherichia coli using the T7 RNA polymerase promotor. The enzyme accounts for approx. 1% of the soluble protein fraction and is expressed as a soluble and active enzyme. Comparison with GAO expressed in Saccharomyces cerevisiae (Macheroux, P., Massey, V., Thiele, D.J. and Volokita, M. (1991) Biochemistry 30, 4612-4619) showed that the GAO expressed in E. coli has identical physico-chemical features to the wild-type enzyme, but is expressed at a level approx. 15-fold higher than in the yeast system.

引用

页码：11 / 16

页数：6

共 18 条

[1] PHENOTYPIC REVERSAL IN DAM MUTANTS OF ESCHERICHIA-COLI K-12 BY A RECOMBINANT PLASMID CONTAINING THE DAM+ GENE [J].