IDENTIFICATION OF THE 2ND MAJOR ALLERGEN OF JAPANESE CEDAR POLLEN

被引：136

作者：

SAKAGUCHI, M

INOUYE, S

TANIAI, M

ANDO, S

USUI, M

MATUHASI, T

机构：

[1] HAYASHIBARA BIOCHEM LABS INC,OKAYAMA,JAPAN

[2] OKINAKA MEM INST MED RES,TOKYO,JAPAN

来源：

ALLERGY | 1990年 / 45卷 / 04期

关键词：

ELISA; IgE antibody; Japanese cedar pollen; major allergen; N‐terminal amino acid sequence; pollinosis;

D O I：

10.1111/j.1398-9995.1990.tb00501.x

中图分类号：

R392 [医学免疫学];

学科分类号：

100102 ;

摘要：

We isolated and characterized the second major allergen (Cry j II) from Japanese cedar pollen. We found that most patients with this pollinosis had IgE antibody to this protein in addition to IgE antibody to Cry j I; however, some sera reacted only with Cry j I or Cry j IL IgE‐ELISA inhibition studies revealed that Cry j I and Cry j II had no cross‐allergenicity. Cry j II did not react with anti‐Cry j I monoclonal antibodies. In SDS‐PAGE under a non‐reducing condition. Cry j II showed a band at the 37 kDa position, compared with the 45–50 kDa bands of Cry j I. N‐terminal amino acid sequence of Cry j II was completely different from that oi Cry j I. Copyright © 1990, Wiley Blackwell. All rights reserved

引用

页码：309 / 312

页数：4

共 9 条

[1] ISOLATION AND PARTIAL CHARACTERIZATION OF ALLERGEN IN MOUNTAIN CEDAR POLLEN [J].