Detection of genetically engineered plants by polymerase chain reaction (PCR) using the FLAVR SAVR(TM) tomato as an example

被引：64

作者：

Meyer, R

机构：

来源：

ZEITSCHRIFT FUR LEBENSMITTEL-UNTERSUCHUNG UND-FORSCHUNG | 1995年 / 201卷 / 06期

关键词：

D O I：

10.1007/BF01201590

中图分类号：

TS2 [食品工业];

学科分类号：

0832 ;

摘要：

In this study, a practical approach for the detection of a genetically engineered tomato is demonstrated by polymerase chain reaction (PCR) assays. The FLAVR SAVR(TM) tomato available on the market which was used as an example, contains a resistance gene for the antibiotic kanamycin (kan(r)) and a gene construct for the inhibition of fruit ripening and softening (antisense polygalacturonase). The presence of these DNA's could only be detected in the genetically engineered tomato.

引用

收藏

页码：583 / 586

页数：4

相关论文

共 26 条

[1] POLYMERASE CHAIN-REACTION (PCR) FOR DETECTION OF PATHOGENIC MICROORGANISMS IN BACTERIOLOGICAL MONITORING OF DAIRY-PRODUCTS [J].

ALLMANN, M ;

HOFELEIN, C ;

KOPPEL, E ;

LUTHY, J ;

MEYER, R ;

NIEDERHAUSER, C ;

WEGMULLER, B ;

CANDRIAN, U .

RESEARCH IN MICROBIOLOGY, 1995, 146 (01) :85-97

[2] POLYMERASE CHAIN-REACTION (PCR) - A POSSIBLE ALTERNATIVE TO IMMUNOCHEMICAL METHODS ASSURING SAFETY AND QUALITY OF FOOD - DETECTION OF WHEAT CONTAMINATION IN NON-WHEAT FOOD-PRODUCTS [J].

ALLMANN, M ;

CANDRIAN, U ;

HOFELEIN, C ;

LUTHY, J .

ZEITSCHRIFT FUR LEBENSMITTEL-UNTERSUCHUNG UND-FORSCHUNG, 1993, 196 (03) :248-251

[3] STRUCTURE AND EVOLUTION OF THE INTERGENIC REGION IN A RIBOSOMAL DNA REPEAT UNIT OF WHEAT [J].

BARKER, RF ;

HARBERD, NP ;

JARVIS, MG ;

FLAVELL, RB .

JOURNAL OF MOLECULAR BIOLOGY, 1988, 201 (01) :1-17

[4] EXCHANGE OF GENE ACTIVITY IN TRANSGENIC PLANTS CATALYZED BY THE CRE-LOX SITE-SPECIFIC RECOMBINATION SYSTEM [J].

BAYLEY, CC ;

MORGAN, M ;

DALE, EC ;

OW, DW .

PLANT MOLECULAR BIOLOGY, 1992, 18 (02) :353-361

[5] NUCLEOTIDE-SEQUENCE AND EXACT LOCALIZATION OF THE NEOMYCIN PHOSPHOTRANSFERASE GENE FROM TRANSPOSON TN5 [J].

BECK, E ;

LUDWIG, G ;

AUERSWALD, EA ;

REISS, B ;

SCHALLER, H .

GENE, 1982, 19 (03) :327-336

[6] POLYMERASE CHAIN-REACTION USED FOR MONITORING MULTIPLE GENE INTEGRATION IN AGROBACTERIUM-MEDIATED TRANSFORMATION [J].

BLAKE, NK ;

DITTERLINE, RL ;

STOUT, RG .

CROP SCIENCE, 1991, 31 (06) :1686-1688

[7]

CANDRIAN U, 1991, Mitteilungen aus dem Gebiete der Lebensmitteluntersuchung und Hygiene, V82, P539

[8]

Candrian Urs, 1994, Mitteilungen aus dem Gebiete der Lebensmitteluntersuchung und Hygiene, V85, P704

[9] EFFICIENT TRANSFER OF A GLYPHOSATE TOLERANCE GENE INTO TOMATO USING A BINARY AGROBACTERIUM-TUMEFACIENS VECTOR [J].

FILLATTI, JJ ;

KISER, J ;

ROSE, R ;

COMAI, L .

BIO-TECHNOLOGY, 1987, 5 (07) :726-730

[10] THE COMPLETE NUCLEOTIDE-SEQUENCE OF AN INFECTIOUS CLONE OF CAULIFLOWER MOSAIC-VIRUS BY M13MP7 SHOTGUN SEQUENCING [J].

GARDNER, RC ;

HOWARTH, AJ ;

HAHN, P ;

BROWNLUEDI, M ;

SHEPHERD, RJ ;

MESSING, J .

NUCLEIC ACIDS RESEARCH, 1981, 9 (12) :2871-2888