IMMUNOHISTOCHEMICAL DETECTION OF THE EPIDERMAL GROWTH-FACTOR RECEPTOR IN PARAFFIN-EMBEDDED HUMAN TISSUES

被引：35

作者：

GULLICK, WJ ^{[1
]}

HUGHES, CM ^{[1
]}

MELLON, K ^{[1
]}

NEAL, DE ^{[1
]}

LEMOINE, NR ^{[1
]}

机构：

[1] UNIV NEWCASTLE UPON TYNE,DEPT SURG UROL,NEWCASTLE TYNE NE1 7RU,TYNE & WEAR,ENGLAND

来源：

JOURNAL OF PATHOLOGY | 1991年 / 164卷 / 04期

关键词：

EPIDERMAL GROWTH FACTOR RECEPTOR; TGF-ALPHA; IMMUNOHISTOCHEMISTRY; BLADDER CANCER; C-ERBB-2; PROTEIN;

D O I：

10.1002/path.1711640403

中图分类号：

R73 [肿瘤学];

学科分类号：

100214 ;

摘要：

Expression of the epidermal growth factor (EGF) receptor was evaluated by immunohistochemical staining of formalin-fixed, paraffin-embedded tumour tissues employing two antibodies raised to short synthetic peptides from the cytoplasmic domain of the molecule. Both antibodies gave concordant staining of a series of bladder cancers known to express or lack EGF receptors. There was no cross-reaction with the related c-erbB-2 protein, which was also overexpressed in some cases. Cancers with EGF receptor expression also expressed high levels of TGF-alpha, a receptor agonist.

引用

页码：285 / 289

页数：5

共 21 条

[1]

BARTON CM, 1991, J PATHOL, V163, P111, DOI 10.1002/path.1711630206

[2] STOCHASTIC APPEARANCE OF MAMMARY-TUMORS IN TRANSGENIC MICE CARRYING THE MMTV/C-NEU ONCOGENE [J].

BOUCHARD, L ;

LAMARRE, L ;

TREMBLAY, PJ ;

JOLICOEUR, P .

CELL, 1989, 57 (06) :931-936

[3] ERBB-2 IS A POTENT ONCOGENE WHEN OVEREXPRESSED IN NIH/3T3 CELLS [J].

DIFIORE, PP ;

PIERCE, JH ;

KRAUS, MH ;

SEGATTO, O ;

KING, CR ;

AARONSON, SA .

SCIENCE, 1987, 237 (4811) :178-182

[4]

DIMARCO E, 1989, ONCOGENE, V4, P831

[5] ANTIBODIES TO THE AUTOPHOSPHORYLATION SITES OF THE EPIDERMAL GROWTH-FACTOR RECEPTOR PROTEIN-TYROSINE KINASE AS PROBES OF STRUCTURE AND FUNCTION [J].

GULLICK, WJ ;

DOWNWARD, J ;

WATERFIELD, MD .

EMBO JOURNAL, 1985, 4 (11) :2869-2877

[6] PREVALENCE OF ABERRANT EXPRESSION OF THE EPIDERMAL GROWTH-FACTOR RECEPTOR IN HUMAN CANCERS [J].

GULLICK, WJ .

BRITISH MEDICAL BULLETIN, 1991, 47 (01) :87-98

[7] EXPRESSION OF THE C-ERBB-2 PROTEIN IN NORMAL AND TRANSFORMED-CELLS [J].

GULLICK, WJ ;

BERGER, MS ;

BENNETT, PLP ;

ROTHBARD, JB ;

WATERFIELD, MD .

INTERNATIONAL JOURNAL OF CANCER, 1987, 40 (02) :246-254

[8]

GULLICK WJ, 1989, MOL BIOL CANCER, P38

[9] THE PROTEIN-KINASE FAMILY - CONSERVED FEATURES AND DEDUCED PHYLOGENY OF THE CATALYTIC DOMAINS [J].

HANKS, SK ;

QUINN, AM ;

HUNTER, T .

SCIENCE, 1988, 241 (4861) :42-52

[10] AN IMMUNOHISTOCHEMICAL STUDY OF THE PRESENCE OF C-ERBB-2 PROTEIN IN PAGETS-DISEASE OF THE NIPPLE [J].

LAMMIE, GA ;

BARNES, DM ;

MILLIS, RR ;

GULLICK, WJ .

HISTOPATHOLOGY, 1989, 15 (05) :505-514

← 1 2 3 →