THE EFFECT OF OSMOTIC CHALLENGE AND SUBSEQUENT REHYDRATION ON THE AGING HYPOTHALAMO-NEUROHYPOPHYSEAL SYSTEM - A QUANTITATIVE MORPHOLOGICAL-STUDY OF THE SUPRAOPTIC NUCLEUS

The neurons of the supraoptic nucleus (SON) of male C57BL/Icrfat mice at 6 or 28 mo. of age were examined from normally hydrated, osmotically loaded and osmotically loaded/rehydrated animals. Using quantitative morphological techniques it was shown that the majority of the ultrastructural variables investigated were controlled in the same way in the SON neurons of young and old mice. The major exception was the mitochondrial compartment which, although maintaining a constant proportion of the volume of the SON cell, did show a significant reduction in the number of mitochondria in the old group, particularly during the osmotic challenge period of the experiment. The "lipid" body compartment of the SON neuron also behaved differently in the cells from the older age group. These SON neuroendocrine neurons from old animals apparently are able to produce hormone until late in the life-span. Other aspects of cellular activity appeared to be altered when judged by morphological criteria. The SON neurons from these old mice are able to synthesize hormone-containing organelles to the same concentration as the cells from a younger animal. The efficiency of the process, or at least the efficiency of concomitant cellular processes, must be questioned in view of the alterations in the rough endopolasmic reticulum, mitochondrial and lysosomal systems.