2 NOVEL MOLECULAR ISOFORMS OF BAND-4.2 IN JAPANESE SIKA DEER (CERVUS-NIPPON-YESOENSIS, HEUDE) ERYTHROCYTES

Two molecular isoforms of band 4.2 were identified in erythrocyte membranes from 25 Japanese Sika deer (Ceryus nippon yesoensis, Heude) based on specific immunorecognition with anti-human band 4.2. These two variants, designated 4.2/78 and 4.2/76, had respective relative molecular weights (Mr of 78 000 and 76 000 on sodium dodecyl sulfate-polyacrylamide electrophoresis gels and showed similar profiles after limited proteolysis, exhibiting identity in primary structure. 25 adult Sika deer could be divided into two groups according to the 4.2/78:42/76 ratio, indicating a genetic control in the epxression of the molecular isoforms of band 4.2. Both polypeptides were completely retained in cytoskeletal protein-depleted membranes and bne removed by alkaline extraction, suggesting that both proteins contribute to the association of membrane proteins. © 1990.