FORMYLMETHANOFURAN - TETRAHYDROMETHANOPTERIN FORMYLTRANSFERASE FROM METHANOSARCINA-BARKERI - IDENTIFICATION OF N5-FORMYLTETRAHYDROMETHANOPTERIN AS THE PRODUCT

被引：32

作者：

BREITUNG, J

THAUER, RK

机构：

[1] Laboratorium für Mikrobiologie, Fachbereich Biologie, Philipps-Universität Marburg, Karl-von-Frisch-Straße

来源：

FEBS LETTERS | 1990年 / 275卷 / 1-2期

关键词：

Formylmethanofuran; Formyltetrahydromethanopterin; Methanobacterium thermoautotrophicum; Methanofuran; Methanosarcina barkeri; Tetrahydromethanopterin;

D O I：

10.1016/0014-5793(90)81477-6

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Formylmethanofuran: tetrahydromethanopterin formyltransferase was purified from methanol grown Methanosarcina barkeri to apparent homogeneity and characterized with respect to its molecular and kinetic properties. The enzyme was found to be very similar to the formyltransferase from H2/CO2 grown Methanobacterium thermoautotrophicum. It also catalyzed the formation of N5-formyltetrahydromethanopterin rather than of N10-formyltetrahydromethanopterin from formylmethanofuran and tetrahydromethanopterin. © 1990.

引用

页码：226 / 230

页数：5

共 16 条

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