THE EFFECT OF SPECIFIC GROWTH-RATE AND DEATH RATE ON MONOCLONAL-ANTIBODY PRODUCTION IN HYBRIDOMA CHEMOSTAT CULTURES

被引：37

作者：

LINARDOS, TI

KALOGERAKIS, N

BEHIE, LA

LAMONTAGNE, LR

机构：

[1] UNIV CALGARY,PHARMACEUT PROD RES FACIL,CALGARY T2N 1N4,ALBERTA,CANADA

[2] CHEMBIOMED LTD,EDMONTON,ALBERTA,CANADA

来源：

CANADIAN JOURNAL OF CHEMICAL ENGINEERING | 1991年 / 69卷 / 02期

关键词：

KINETIC MODELING; MONOCLONAL ANTIBODIES; ANTIBODY PRODUCTION; DEATH RATE; HYBRIDOMA; CHEMOSTAT CULTURES;

D O I：

10.1002/cjce.5450690205

中图分类号：

TQ [化学工业];

学科分类号：

0817 ;

摘要：

Experimental data collected from bench-scale chemostat cultures of mouse-mouse hybridoma cells have been used for the development of a kinetic model of monoclonal antibody production. The specific antibody productivity was found to be proportional to the specific death rate, indicating a stimulating effect of stress on the ability of the cells to produce antibody. The death rate was found to be an exponential function of the average cell age in the culture. Furthermore, a Generalized Maintenance Energy (GME) model is proposed, to take account of the effects of culture stress on the nutrient uptake rates. The proposed relationships agreed very well with other mouse-mouse hybridoma chemostat culture data in the literature.

引用

页码：429 / 438

页数：10

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