DETERMINATION OF SELENIUM FRESH FISH FROM SOUTHEASTERN SPAIN FOR CALCULATION OF DAILY DIETARY-INTAKE

Hydride generation atomic absorption spectrometry was used to determine the total Se content in the edible. muscular tissue of the 31 most common species of fresh fish from the Mediterranean area of Motril (southeastern Spain). The samples were digested in nitric acid. The Se6+ in the digested solutions was prereduced to Se4+ by exothermic reaction in HCl solution. Determination of selenium was carried out by the standard addition method; Results for standard reference material (BCR mussel tissue) agreed closely with the certified values. Detection limit was 2.83 ng. Reproducibility expressed as relative standard deviation ranged from 4.00% to 4.71% A summary of results is reported ranging from 0.150 to 2.014 mu g/g for 31 species of fish. Considering the average daily individual consumption of fresh fish in Andalusia (southern Spain), the daily dietary intake of Se supplied by this source is 15.78 mu g/day for an adult.