COPPER, ZINC SUPEROXIDE-DISMUTASE IN ESCHERICHIA-COLI - PERIPLASMIC LOCALIZATION

被引：59

作者：

BENOV, L

CHANG, LY

DAY, B

FRIDOVICH, I

机构：

[1] DUKE UNIV,MED CTR,DEPT BIOCHEM,DURHAM,NC 27710

[2] DUKE UNIV,MED CTR,DEPT MED,DURHAM,NC 27710

来源：

ARCHIVES OF BIOCHEMISTRY AND BIOPHYSICS | 1995年 / 319卷 / 02期

关键词：

SUPEROXIDE DISMUTASE; CU; ZN; ESCHERICHIA COLI; ZNSOD; PERIPLASMIC CU; LOCALIZATION OF ESCHERICHIA-COLI CU; IMMUNOGOLD LABELING OF CU; ANTIBODY TO ESCHERICHIA-COLI CU;

D O I：

10.1006/abbi.1995.1324

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Cu,ZnSOD purified from Escherichia coli has been used to raise antibodies in rabbits. The resultant antiserum was found to recognize a single band on Western blots of SDS-polyacrylamide gel electropherograms, and that single band coincided with the position of the Cu,ZnSOD, Ultrathin sections of fixed E. coli were treated with the antibody followed by protein A bearing 10-nm gold particles. Electron microscopy revealed that Cu,ZnSOD was largely localized in the periplasm in polar bays. (C) 1995 Academic Press, Inc.

引用

页码：508 / 511

页数：4

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