FURTHER-STUDIES ON PARTIALLY PURIFIED CALF THYMUS DNA POLYMERASE-ALPHA

Attempts to prevent the urea conversion of a 200-230, 000 molecular weight DNA polymerase a to a 150-170,000 molecular weight form by the inclusion of protease inhibitors have not been successful. No other method has been found capable of dissociating a 50-70,000 fragment or subunit from the DNA polymerase subunit. Addition of this 50-70,000 subunit to the polymerase subunit does not aid the binding of the enzyme to DNA, but does have an effect on the utilisation of synthetic template-initiator complexes by the polymerase subunit. © 1979 Information Retrieval Limited.