SUBCELLULAR-LOCALIZATION OF THE ENZYME THAT FORMS MANNOSYL RETINYL PHOSPHATE FROM GUANOSINE DIPHOSPHATE [MANNOSE-C-14 AND RETINYL PHOSPHATE

被引：16

作者：

SMITH, MJ

SCHREIBER, JB

WOLF, G

机构：

来源：

BIOCHEMICAL JOURNAL | 1979年 / 180卷 / 03期

关键词：

D O I：

10.1042/bj1800449a

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

The subcellular distribution of the enzyme catalysing the conversion of retinyl phosphate and GDP-[14C]mannose into [14C]mannosyl retinyl phosphate was determined by using subcellular fractions of rat liver. Purity of fractions, as determined by marker enzymes, was 80% or better. The amount of mannosyl retinyl phosphate formed (pmol/min per mg of protein) for each fraction was: rough endoplasmic reticulum 0.48 +/- 0.09 (mean +/- S.D.); smooth membranes (consisting of 60% smooth endoplasmic reticulum and 40% Golgi apparatus), 0.18 +/- 0.03; Golgi apparatus, 0.13 +/- 0.03; and plasma membrane 0.02.

引用

页码：449 / 453

页数：5

共 17 条

[1] CELL-SURFACE MANNOSYL TRANSFERASE-ACTIVITY IN LIVER OF EMBRYONIC CHICK - MANNOSE CONTAINING GLYCOLIPID AS INTERMEDIATE IN GLYCOPROTEIN SYNTHESIS [J].