LEVANSUCRASE - A TOOL TO STUDY PROTEIN SECRETION IN BACILLUS-SUBTILIS

被引：8

作者：

NAGARAJAN, V

BORCHERT, TV

机构：

[1] Central Research and Development Division, E.I. DuPont de Nemours Inc, Wilmington

来源：

RESEARCH IN MICROBIOLOGY | 1991年 / 142卷 / 7-8期

关键词：

BACILLUS-SUBTILIS; LEVANSUCRASE; SECRETION; SELECTION SYSTEM;

D O I：

10.1016/0923-2508(91)90056-G

中图分类号：

Q93 [微生物学];

学科分类号：

071005 ; 100705 ;

摘要：

The Bacillus amyloliquefaciens levansucrase gene (sacB[BamP]) was engineered in such a way that a heterologous gene could be inserted between the second and third codon of the mature levansucrase. Extracellular levansucrase activity was detected only when the heterologous protein was secreted into the growth medium. A positive selection system to isolate suppressors of signal sequence mutants in Bacillus subtilis has been developed based on the secretion of levansucrase.

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页码：787 / 792

页数：6

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