PROTEIN-KINASE-C DEPENDENT AND INDEPENDENT ACTIVATION OF PHOSPHOLIPASE-A2 UNDER CALCIUM IONOPHORE (A23187) EXPOSURE IN RABBIT PULMONARY ARTERIAL SMOOTH-MUSCLE CELLS

被引：26

作者：

CHAKRABORTI, S ^{[1
]}

MICHAEL, JR ^{[1
]}

PATRA, SK ^{[1
]}

机构：

[1] UNIV UTAH, HLTH SCI CTR, DEPT MED, SALT LAKE CITY, UT 84132 USA

来源：

FEBS LETTERS | 1991年 / 285卷 / 01期

关键词：

CALCIUM IONOPHORE; PHOSPHOLIPASE-A2; ARACHIDONIC ACID; SPHINGOSINE; PROTEIN KINASE-C; SMOOTH MUSCLE CELL; CALCIUM SIGNAL;

D O I：

10.1016/0014-5793(91)80735-L

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Exposure of rabbit pulmonary arterial smooth muscle cells to the calcium ionophore A23187, dose-dependently stimulates arachidonic acid (AA) release and phospholipase A2 (PLA2) activity. The protein kinase C (PKC) inhibitor, sphingosine does not prevent AA release and PLA2 activity caused by low doses of A23187. In contrast, sphingosine markedly prevents AA release and PLA2 activity caused by higher doses of A23187. PKC activity profile indicates that treatment of the cells with low doses of A23187 does not cause significant alteration of PKC translocation from cytosol to membrane whereas higher concentrations of the ionophore dose-dependently enhance PKC translocation from cytosol to membrane in the smooth muscle cells.

引用

页码：104 / 107

页数：4

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