BISTABILITY AND REACTION THRESHOLDS IN THE PHENOL-INHIBITED PEROXIDASE-CATALYZED OXIDATION OF INDOLE-3-ACETIC-ACID

被引：10

作者：

KRYLOV, SN

AGUDA, BD

LJUBIMOVA, ML

机构：

[1] LAURENTIAN UNIV,DEPT CHEM & BIOCHEM,SUDBURY,ON P3E 2C6,CANADA

[2] MOSCOW MV LOMONOSOV STATE UNIV,INST NUCL PHYS,MOSCOW 119899,RUSSIA

来源：

BIOPHYSICAL CHEMISTRY | 1995年 / 53卷 / 03期

基金：

加拿大自然科学与工程研究理事会;

关键词：

OXIDATION OF INDOLE-3-ACETIC ACID; PHENOL INHIBITION MECHANISM; REACTION THRESHOLD; BISTABILITY; MODELING; COMPUTER SIMULATION;

D O I：

10.1016/0301-4622(94)00106-T

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

A model mechanism for the phenol-inhibited peroxidase-catalyzed oxidation of indole-3-acetic acid (IAA) is proposed and analyzed. The model involves an autocatalytic free radical species that sustains IAA oxidation and the phenolic inhibitor acting as a free radical scavenger. Under a fixed set of parameter values, the model exhibits a coexistence of two stable steady states. This bistability phenomenon explains the origin of the experimentally observed threshold inhibitor concentrations above which IAA oxidation stops. The variation of the inhibitor threshold level with enzyme and substrate concentrations are reproduced by the model almost quantitatively.

引用

页码：213 / 218

页数：6

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