BRAIN DISTRIBUTION OF 4 RAT HOMOLOGS OF THE DROSOPHILA DUNCE CAMP-PHOSPHODIESTERASE

被引：48

作者：

ENGELS, P

ABDELAL, S

HULLEY, P

LUBBERT, H

机构：

[1] SANDOZ PHARMA LTD, PRECLIN RES 386226, CH-4002 BASEL, SWITZERLAND

[2] SANDOZ RES INST, BERN, SWITZERLAND

来源：

JOURNAL OF NEUROSCIENCE RESEARCH | 1995年 / 41卷 / 02期

关键词：

IN SITU HYBRIDIZATION; HIPPOCAMPUS; OLFACTORY BULB; LTP; MEMORY;

D O I：

10.1002/jnr.490410204

中图分类号：

Q189 [神经科学];

学科分类号：

071006 ;

摘要：

We have analyzed the brain distribution of the rat cAMP-specific phosphodiesterases (rPDEIV) which are closely related to the defective gene products of the drosophila melanogaster learning and memory mutant dunce, PCR analysis of rat brain cDNA was performed on the four known dunce-like cAMP PDE rat isogenes (rPDE-IV-A, -B, -C, -D). High expression of three of these isogenes (rPDEIV-A, -B, -D) highlighted their involvement in regulation of cAMP in the brain, Specific probes for all four isogenes were then used for in situ hybridization of rat brain sections. Distinct but overlapping expression patterns were observed for rPDEIV-A, rPDEIV-B, and rPDEIV-D, Abundant expression of these subtypes was observed in the olfactory system, the hippocampus and the cerebellum, while no specific signals could be detected in most areas of the brain for the subtype rPDEIV-C. (C) 1995 Wiley-Liss, Inc.

引用

页码：169 / 178

页数：10

共 54 条

[1]

Barrionuevo G, Brown TH, Associative long‐term potentiation in hippocampal splices, Proc Natl Acad Sci USA, 80, pp. 7347-7351, (1983)

[2]

Bartsch S, Bartsch U, Dories U, Faissner A, Weller A, Ekblom P, Schachner M, Expression of tenascin in the developing and adult cerebellar cortex, J Neurosci, 12, pp. 736-749, (1992)

[3]

Beavo JA, Reifsnyder DH, Primary sequence of cyclic nucleotide phosphodiesterase isozymes and the design of selective inhibitors, Trends Pharm Sci, 11, pp. 150-155, (1990)

[4]

Bolger G, Michaeli T, Martins T, St. John T, Steiner B, Rodgers L, Riggs M, Wigler M, Ferguson K, A family of human phosphodiesterases homologous to the dunce learning and memory gene product of Drosophila melanogaster are potential targets for antidepressant drugs, Mol Cell Biol, 13, pp. 6558-6571, (1993)

[5]

Breipohl W, Mackay-Sim A, Grandt D, Rehn D, Darrelmann C, Ontogeny of Olfaction, pp. 21-34, (1986)

[6]

Browning MD, Dudek EM, Activators of protein kinase C increase the phosphorylation of synapsin at sites phosphorylated by cAMP‐dependent and Ca<sup>2+</sup>/calmodulin‐dependent protein kinases in the rat hippocampus slice, Synapse, 10, pp. 62-70, (1992)

[7]

Byers D, Davis RL, Kiger JA, Defect in cyclic AMP phosphodiesterase due to the dunce mutation of learning in Drosophila melanogaster, Nature, 289, pp. 79-81, (1981)

[8]

Chen CN, Denome S, Davis RL, Molecular analysis of cDNA clones and the corresponding genomic coding region of the drosophila dunce + locus, the structural gene for cAMP phosphodiesterase, Proc Natl Acad Sci USA, 86, pp. 3599-3603, (1986)

[9]

Chomczynski P, Sacchi N, Single step method of RNA isolation by acid guanidinium thiocyanate‐phenol‐chloroform extraction, Analyt Biochem, 162, pp. 156-159, (1987)

[10]

Colicelli J, Birchmeier C, Michaeli T, O'Neill K, Riggs M, Wigler M, Isolation and characterization of a mammalian gene encoding a high‐affinity cAMP phosphodiesterase, Proc Natl Acad Sci USA, 86, pp. 3599-3603, (1989)

← 1 2 3 4 5 6 →