PURIFICATION AND THE HISTONES OF DICTYOSTELIUM-DISCOIDEUM CHROMATIN

被引：14

作者：

BAKKE, AC ^{[1
]}

BONNER, J ^{[1
]}

机构：

[1] CALTECH,DIV BIOL,PASADENA,CA 91125

来源：

BIOCHEMISTRY | 1979年 / 18卷 / 21期

关键词：

D O I：

10.1021/bi00588a016

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Dictyostelium chromatin has been purified from nuclei in high yield by differential centrifugation and nuclease cleaving. Its chemical composition has been assayed, and its histones have been analyzed by gel electrophoresis, peptide fingerprints, amino acid composition, and ion-exchange chromatography. The mass ratios of DNA/RNA/histone/ nonhistone are 1.0:0.18:0.98:1.02. There are four histones including one unusual histone, H7, which is the most abundant histone in the slime mold. The H4-like protein is the most conserved protein, while the other histones show both similarities and differences with mammalian histones. © 1979, American Chemical Society. All rights reserved.

引用

页码：4556 / 4562

页数：7

共 40 条

[1] CHROMATIN STRUCTURE IN CELLULAR SLIME-MOLD DICTYOSTELIUM-DISCOIDEUM [J].