FLUOROMETRIC-DETERMINATION OF BINDING CONSTANTS BETWEEN MICELLES AND CHEMICAL-SYSTEMS

A fluorimetric method is adapted to determine the binding constants (K) between different systems and micelles. The method is based on the variation of the fluorescence of the system in the presence of increasing amounts of a surfactant agent. This method permits the determination of binding constants in strong acid or alkaline media. The binding constants of 1-naphthol and 2-(4-alkylamino-2 hydroxyphenyl)benzo-X azoles (X being an O or an S atom, PAS-O and PAS-S) with cationic, anionic, and nonionic surfactants have been determined by the proposed method. Values from 49,100 M-1 for the interaction between PAS-S and CTAB in basic medium to 10 M-1 for the interaction of 1-naphthol with a nonylphenol ethylene oxide condensate have been found. © 1991.