INSITU NICK TRANSLATION OF HUMAN METAPHASE CHROMOSOMES WITH THE RESTRICTION ENZYMES MSPI AND HPAII REVEALS AN R-BAND PATTERN

被引：27

作者：

ADOLPH, S ^{[1
]}

HAMEISTER, H ^{[1
]}

机构：

[1] UNIV ULM,KLIN GENET ABT,W-7900 ULM,GERMANY

来源：

CYTOGENETICS AND CELL GENETICS | 1990年 / 54卷 / 3-4期

关键词：

D O I：

10.1159/000132976

中图分类号：

Q2 [细胞生物学];

学科分类号：

071009 ; 090102 ;

摘要：

Several restriction enzymes (HindIII, HaeIII, MspI, HpaII, EcoRI, KpnI, and NotI) were evaluated for their ability to induce bands in human metaphase chromosomes during in situ nick translation. MspI and HpaII were able to induce a completely developed R-band pattern. Preferential cleavage of R-band chromatin is due to the presence of unmethylated CpG-residues present in CpG-rich islands, which are apparently unevenly distributed and mainly concentrated in R-bands.

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页码：132 / 136

页数：5

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