LOW-DENSITY-LIPOPROTEIN ACTIVATES MONOCYTES TO EXPRESS TUMOR-NECROSIS-FACTOR

被引：21

作者：

BARATH, P

CAO, J

FORRESTER, JS

机构：

[1] Cedars-Sinai Medical Center, Division of Cardiology, Los Angeles, CA

来源：

FEBS LETTERS | 1990年 / 277卷 / 1-2期

关键词：

MONOCYTE; TUMOR NECROSIS FACTOR; LOW DENSITY LIPOPROTEIN; GENE EXPRESSION;

D O I：

10.1016/0014-5793(90)80838-A

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

We analyzed the effect of acetylated low density lipoprotein (aLDL) incubation on tumor necrosis factor (TNF) mRNA and protein expression in isolated resting human monocytes in serum free DMEM. TNF mRNA expression was about one third that of PMA and was dose dependent. The maximum stimulatory effect on TNF mRNA was at 250 mu-g/ml, while 500 mu-g/ml induced downregulation. The maximum stimulatory effect occurred at 6 hours, and by 24 hours TNF mRNA expression returned to the resting state. Acetyl LDL also induced the expression of immunoreactive TNF, reaching a sevenfold maximum above control at 12 hours following a 6 hour exposure period. The results suggest that aLDL is a potent stimulator of TNF expression in resting monocytes. This mechanism may be operational in atheroma evolution.

引用

页码：180 / 184

页数：5

共 28 条

[1] METHOD FOR DETECTION OF SPECIFIC RNAS IN AGAROSE GELS BY TRANSFER TO DIAZOBENZYLOXYMETHYL-PAPER AND HYBRIDIZATION WITH DNA PROBES [J].