DEMONSTRATION OF SEROTONIN RECEPTORS IN ISOLATED SPISULA OOCYTE MEMBRANE

被引：22

作者：

BANDIVDEKAR, AH

SEGAL, SJ

KOIDE, SS

机构：

[1] POPULAT COUNCIL,CTR BIOMED RES,1230 YORK AVE,NEW YORK,NY 10021

[2] MARINE BIOL LAB,WOODS HOLE,MA 02543

[3] INST RES REPROD,BOMBAY 400012,INDIA

[4] ROCKEFELLER FDN,NEW YORK,NY 10036

来源：

INVERTEBRATE REPRODUCTION & DEVELOPMENT | 1991年 / 19卷 / 02期

关键词：

SEROTONIN; SPISULA OOCYTES; OOCYTE MEMBRANES; OOCYTE MATURATION; 5-HT RECEPTORS;

D O I：

10.1080/07924259.1991.9672168

中图分类号：

Q [生物科学];

学科分类号：

07 ; 0710 ; 09 ;

摘要：

A method for the isolation of Spisula oocyte membranes was developed. 5-hydroxytryptamine (5-HT) receptors in the isolated oocyte membranes were determined by measuring the binding of [H-3]5-HT. The dissociation constant of [H-3]5-HT binding to the membrane receptor was estimated to be 17.5 nM and the maximum binding capacity was 7.9 pmoles/mg protein. Inhibition study of [H-3]5-HT binding with site selective 5-HT analogs was performed. The results suggest that the 5-HT receptors in oocyte membranes contain sites that interact with 5-HT1 and 5-HT3 selective analogs.

引用

页码：147 / 150

页数：4

共 13 条

[1] CYCLIC-NUCLEOTIDE CONTENT AND PROTEIN-PHOSPHORYLATION DURING MATURATION OF SPISULA OOCYTES [J].