INVITRO STUDIES OF THE BINDING OF THE ARGR PROTEINS TO THE ARG5,6 PROMOTER

被引：47

作者：

DUBOIS, E ^{[1
]}

MESSENGUY, F ^{[1
]}

机构：

[1] CTR ENSEIGNEMENT & RECH IND ALIMENTAIRES & CHIM,INST RECH,1 AVE E GRYSON,B-1070 BRUSSELS,BELGIUM

来源：

MOLECULAR AND CELLULAR BIOLOGY | 1991年 / 11卷 / 04期

关键词：

D O I：

10.1128/MCB.11.4.2162

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

ARGRI, ARGRII, and ARGRIII regulatory proteins control the expression of arginine anabolic and catabolic genes in Saccharomyces cerevisiae. We show here that they are also required in vitro to observe a protein-DNA complex with the promoter of the ARG5,6 gene. The specific binding of ARGR proteins in vitro is stimulated by arginine. Antibodies raised against a synthetic MCM1 polypeptide retard the migration of ARGR-DNA complex on gel mobility shift assays. This result suggests that MCM1 could be an additional regulatory element of arginine metabolism.

引用

页码：2162 / 2168

页数：7

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