EXPANSION OF HUMAN BONE-MARROW PROGENITOR CELLS IN A HIGH CELL-DENSITY CONTINUOUS PERFUSION SYSTEM

被引:83
作者
PALSSON, BO
PAEK, SH
SCHWARTZ, RM
PALSSON, M
LEE, GM
SILVER, S
EMERSON, SG
机构
[1] UNIV MICHIGAN, DEPT INTERNAL MED, HEMATOL ONCOL RES LAB, ANN ARBOR, MI 48109 USA
[2] AASTROM BIOSCI INC, ANN ARBOR, MI 48109 USA
来源
BIO-TECHNOLOGY | 1993年 / 11卷 / 03期
关键词
D O I
10.1038/nbt0393-368
中图分类号
Q81 [生物工程学(生物技术)]; Q93 [微生物学];
学科分类号
071005 ; 0836 ; 090102 ; 100705 ;
摘要
We describe here a continuous perfusion bioreactor system that enables a population of unselected human mononuclear bone marrow-cells obtained from adult donors to expand up to 20 to 25-fold over a two-week period. Colony-forming units of granulocyte-macrophage (CFU-GM) progenitor cells expand 10 to 30-fold. These expansions depend on the gas phase oxygen concentration, the seeding density and time of cell harvest. Under operating conditions that allow for good cell proliferation, 3 to 4 million mononuclear cells can be obtained per square centimeter, with 0.5 to 0.8% being progenitor cells. Autologous human sera supported cell expansion as efficiently as animal sera. Increasing the size of the perfusion system to produce a clinically meaningful number of CFU-GMs could have important applications in bone marrow transplantation therapies.
引用
收藏
页码:368 / 372
页数:5
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