Structure of the even-skipped homeodomain complexed to AT-rich DNA: New perspectives on homeodomain specificity

被引：106

作者：

Hirsch, JA

Aggarwal, AK

机构：

[1] Department of Biochemistry, College of Physicians and Surgeons, Columbia University, New York

来源：

EMBO JOURNAL | 1995年 / 14卷 / 24期

关键词：

crystal structure; even-skipped; homeodomain; protein-DNA; interactions;

D O I：

10.1002/j.1460-2075.1995.tb00318.x

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

even-skipped is a homeobox gene important in controlling segment patterning in the embryonic fruit fly. Its homeobox encodes a DNA binding domain which binds with similar affinities to two DNA consensus sequences, one AT-rich, the other GC-rich. We describe a crystallographic analysis of the Even-skipped homeodomain complexed to an AT-rich oligonucleotide at 2.0 Angstrom resolution, The structure reveals a novel arrangement of two homeodomains bound to one 10 bp DNA sequence in a tandem fashion. This arrangement suggests a mechanism for the homeoproteins' regulatory specificity, In addition, the functionally important residue Gln50 is observed in multiple conformations making direct and water-mediated hydrogen bonds with the DNA bases.

引用

页码：6280 / 6291

页数：12

共 71 条

[1] DIFFERENTIAL DNA-BINDING SPECIFICITY OF THE ENGRAILED HOMEODOMAIN - THE ROLE OF RESIDUE-50 [J].